![]() ![]() ![]() The proposed method represents an affordable, accurate and reproducible approximation that could be used in the presence of limited resources availability.īackground subtraction Densitometry ImageJ Quantification Western blot.Ĭopyright © 2018 Elsevier B.V. In this work, we describe the use of office scanner coupled with the ImageJ software together with a new image background subtraction method for accurate Western blot quantification. Furthermore, the use of semi-quantitative software as ImageJ (Java-based image-processing and analysis software) is clearly increasing in different scientific fields. This dot blot image is available in the File/Open Samples menu in ImageJ 1.33s or later. How to quantify Total protein after Western blot by using ImageJ software Question. Due to the technical knowledge required for densitometry analysis together with the resources availability, standard office scanners are often used for the imaging acquisition of developed Western blot films. ![]() Western blot quantification method constitutes a critical step in order to obtain accurate and reproducible results. It constitutes a multistep method that allows the detection and/or quantification of proteins from simple to complex protein mixtures. The most appropriate method for background correction depends on your image. The first is to treat each row as a horizontal lane and use ImageJs gel analysis function. Since its first description, Western blot has been widely used in molecular labs. Use background subtraction during your analysis to quantify your Western blot data. There are two built in methods for analyzing a dot blot in ImageJ. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |